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According to ED treatment guidelines, oral phosphodiesterase type 5 inhibitors (PDE5 inhibitor) are considered first-line therapy.4 Tadalafil was in phase II trials for the treatment of female sexual dysfunction, however, further investigation was discontinued

emission 520 nm) was read in the upper and lower chambers, and the permeability coefficient was determined using the following formula: 22 P dextran =( RFU lower / RFU upper )( V )(1/ t )(1/ A ), where RFU is the relative fluorescent units in the upper and lower wells, V is the volume of the bottom well, t is the time that the FITC-dextran was allowed to diffuse, and A is the total surface area of the monolayer (cm 2 )

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