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doi: 10.2991/jegh.k.191028.001 2 BommerCSagalovaVHeesemannEManne-GoehlerJAtunRBrnighausenTet al

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Extracts were purified using pH-resistant tC18 cartridges (Waters, Hedehusene, Denmark), dried under a gentle stream of compressed air overnight and reconstituted in 1 ml of TRIS buffer (100 mmol/l TRIS buffer [Sigma-Aldrich] supplemented with 0.1% [wt/vol.] human serum albumin [Merck, Darmstadt, Germany], 20 mmol/l EDTA and 0.6 mmol/l thimerosal [Sigma Chemical Co., St Louis, MO, USA]

Further, investigation of physical signals such as, temperature is also one of the key factors in oncology, which is frequently altered in cancerous condition

189 RicciA.FeliciL.MariottaS.ManninoF.SchmidG.TerzanoC.et al (2004)