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Gene expression levels of Glp1r (Mm00445292_m1), Col1a1 (Mm00801666_g1), Col3a1 (Mm01254476_m1), and Col5a1 (Mm00489299_m1) were calculated as 2 CT relative to the reference gene Rpl32 (Mm02528467_g1)

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However, intermittent fasting lasting less than 48 h did not demonstrate such beneficial effects compared to longer-term caloric restriction 50

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