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[61] They were examining hormones secreted during eating, and testing them on pig pancreases, leading to the discovery of GLP-1's significant effects in 1988
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As such, with 564 semaglutide analogues (supplementary file sema.txt ) in place, a new set of Modigy [28] workflow was conducted with four missense mutations introduced into the peptide backbone of semaglutide, leading to the identfication of a structural electrostatic scaffold at the binding interface of GLP-1R ECD and semaglutide analogue A747 (supplementary file A747.pdb ) with four site-specific missense mutations, namely L23B_R, I20B_Q, V24B_R and R25B_A
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