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The supernatant was discarded, 600 L of 75% ethanol (anhydrous ethanol:DEPC-treated water = 3:1) was added, the precipitate was oscillated, and the precipitate was washed and centrifuged at 12,000 rpm at 4 for 5 min (this process was repeated 3 times)

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Role of amylin in glucose homeostasis

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Moreover, glucagon treatment in human hepatocytes leads to the downregulation of CYP gene expression, and the underlying mechanism may be driven by the activation of glucagon receptors expressed in the liver