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(PDF 2629 kb) Human blood was processed for GSH analysis by HPLC (i.e., by collection into tubes containing K 3 EDTA/NEM310 and deproteination by addition of TCA15 (as described in the PROCEDURE))

Hypothesis 3: Storage temperature will significantly impact levels of total GSH, free GSH, GSSG, and the ratio of free GSH to GSSG

The primary reference is Raun and colleagues (Eur J Endocrinol, 1998), the paper that introduced the molecule and established the property it is known for

This balance was something I had struggled to achieve before