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2 Methionine metabolism Methionine and its metabolites, not only participate in protein synthesis but also have functional roles

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Step 4: draw the bacteriostatic water Using a larger syringe (1 mL or 3 mL with an 18-22 gauge needle), draw air equal to the volume of water you plan to extract

doi: 10.3390/ijms25042294 74 ShiLJuPMengXWangZYaoLZhengMet al

To avoid interferences due to different expression levels of GFP-constructs among cell population, mean fluorescence intensity was measured at the GM130- or MannII-BFP-positive Golgi region as well as at the total cell area, and ratio values were used to determine the relative Golgi accumulation of the constructs