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Because vitamin B12 helps the body produce brain chemicals that can affect mood and cognitive functions, a deficiency may lead to anxiety or depression

But some people might experience improvements in PMS symptoms after losing weight

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)