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Modeling Resistance Mechanisms in Gram-Positive Pathogens
the substrate enables non-invasive monitoring of luciferase-expressing cells (tumor
Dispose of waste and unused material according to institutional
purchase 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740)
Forms a colorless or nearly colorless solution when dissolved 1% in 1M HCl or water
Guanidine thiocyanate (Guanidine Isothiocyanate) 58186279 Modeling Resistance Mechanisms in Gram-PositiveDuring cell lysis and the extraction of DNA or RNA, denaturing enzymes such as DNase and RNase are released. To prevent DNA or RNA denaturation, the chaotropic agent, Guanadine thiocyanate is the preferred agent for use during cell lysis, and for DNA or RNA extraction. This is because of its strong ability to irreversibly inhibit the activity of DNase and RNase. Guanadine thiocyanate has also been effective in deactivating enveloped viruses; however,
