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OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole

10.1016/j.heliyon.2023.e21950 140 WangA.GuanB.YuL.LiuQ.HouY.LiZ.et al (2024a)

Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis