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Agrobacterium transformation of Arabidopsis thaliana roots: A quantitative assay
MF: C15H19NO6
1-O-Methyl-α- D glucopyranoside
and buffering conditions (pH
Lichtenberg
4-Methylumbelliferyl phosphate, free acid Size:10 g Agrobacterium transformation of Arabidopsis thaliana4 Methylumbelliferyl phosphate (MUP) is a sensitive fluorogenic substrate for fluorometric and UV spectrophotometric assays of phosphatase. Phosphotase activity has long been studied due to its importance in cellular function. Upon dephosphorylation, MUP yields fluorescent 4 methylumelliferone (4 MU). This reaction has been used to spot PCR amplified products in ELISA and to classify bacteria. More specifically, MUP can act as an indicator of C.
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