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For the reduction of roGFP2, 2 M diamide oxidized roGFP2 WT or 2 M glutathionylated roGFP2 C151S or roGFP2 C208S in syringe 1 were mixed with variable concentrations of reduced PfGrx and GSH with 0.4 U/mL GR and 0.25 mM NADPH in syringe 2