chewing tobacco in denmark Copenhagen (tobacco) Nicotine Pouch Flavour Bans in

doi: 10.1136/bmjopen-2020-039918 22

This damages your: From your blood, nicotine heads to your brain

14 One millilitre of urine was taken in a test tube to which 2ml of reagent (15% w/v TCA, 0.375% w/v TBA and 0.25N HCl) was added and kept in boiling water bath for 15 min and the contents were allowed to cool and then centrifuged at 1000g for 10 min

Acknowledgements The authors would like to thank Mark Tanner and Jonathan Howard for their invaluable help and expertise in the early stages of this study, and would also like to extend thanks to the Imanova Centre for Imaging Sciences for general support of the work

nikkansports.com (in Japanese)