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The cellular edema that occurs is followed by vasogenic edema that worsens the damage, and overall, the phenomenon is observed as BBB opening (Unterberg et al., 2004)

We observed that the number of endothelial cells, stained by von Willebrand Factor (vWF), a widely used biomarker for vascular endothelial cells, was significantly diminished in ENDS-exposed mice compared with the control mice ( Figures 4C, D )