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To determine whether apoptosis induced by SLC25A39 deficiency was ROS-dependent, cells were treated with the ROS scavenger N-acetyl-L-cysteine (NAC)

In parallel experiments, PDHA1 knockdown markedly enhanced the sensitivity of PCa cells to Elesclomol (ES), a potent inducer of cuproptosis, exerting a more pronounced cytotoxic effect than Enz

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Ethical approval All animal-related procedures were conducted in accordance with the institutional ethical guidelines for the care and use of laboratory animals, following approval from the Institutional Animal Ethics Committee (IAEC) of Banasthali Vidyapith, Rajasthan, under approval number BV/IAEC/September2023/10