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Six test subjects -- all men who were researchers involved with the study -- spent five hours at a time in the chamber while wearing a breathing hood that supplied clean air

This restriction decreases blood flow, starving your skin of oxygen and essential nutrients

Finally, the animals were euthanized at the end of the experiment under deep anesthesia by intraperitoneal injection of ketamine/xylazine followed by intravenous injection of KCL (149 mg/ml, 0.5 ml/250 gm body weight) to stop the hearts in a relatively diastolic or relaxed state

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