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Before the stimulation, 15 l of NbLumSyt1-HALO was premixed with 3 l of Janelia Fluor 646 HaloTag ligand (100 pmol of JF646 stock at 33 M, Promega GA112A) for 15 min at RT and diluted to 300 l of final volume in Tyrode (to reach 4.85 M final concentration of NbLumSyt1-HALO-JF646)

PT-141 can be prescribed off-label for men based on the safety and efficacy trials that have been completed