This discrepancy arises because urban studies typically focus on sampling cigarette butts directly, whereas aquatic assessments usually characterize the entire litter composition and quantify cigarette butts as part of these assemblages
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Systematic analyses of different assemblage-level monitoring data have revealed approximately balanced positive and negative trends in species richness, total abundance 6,8,23,24 , and population trajectories 25,26,27,28 , alongside strong evidence for widespread changes in species composition 6,8,23,29,30