Enzyme Activities For enzyme activities, cells and mouse tissues (duodenum, liver, spleen) were homogenized with a TissueLyser II (Qiagen, Hilden, Germany) in Tris buffer (100 mM Tris (Carl Roth), 300 mM KCl (Applichem, Darmstadt, Germany), pH 7.6 with 0.1% ( v/v ) Triton X-100 (Serva, Heidelberg, Germany), and 0.1% ( v/v ) protease inhibitor (Merck/Millipore, Burlington, MA, USA)) two times for 60 s at 30 Hz
Martin KR, Coles KM
For evaluation of the binding affinity, the antigen structures were first obtained from RCSB protein data bank
2008;31 Suppl 1:S61-78
Systemic Benefits: Proper absorption supports liver health, immune function, and skin radiance