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Autosomal recessive mutations in the genes parkin RBR E3 ubiquitin protein ligase ( PRKN ) and PTEN-induced kinase 1 ( PINK1 ) make up the majority of inherited early-onset PD cases
Immunofluorescence Immunofluorescence staining was performed according to the standard protocol described previously ( Apoptosis Assay and TUNEL Assay Propidium iodide (PI) and Annexin V-FITC-flow cytometry assay (BD Pharmingen, CA) was used to detect the apoptosis in the cells
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Najmi et al., 2022
Some subjects experience declining response a Phase 2 trial found IGF-1 levels plateaued at month 6 despite continued daily MK-677 dosing, suggesting partial desensitization of the GH-IGF-1 axis under constant stimulation
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