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Cells were lysed using mild lysis buffer [20 mM tris at pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 10% glycerol, 1 protease inhibitor cocktail, and 1 phosphatase inhibitor (Roche)]

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While the government, public health officials, tobacco companies and others share a responsibility to provide tobacco consumers with accurate information about the various health risks associated with smoking the goal of informing the public about the risks of tobacco use can and should be accomplished consistent with the U.S