Understanding this distinction is the key to making an informed decision for your research model
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Point mutations in ROGFP2 WT -PFGRX C32S/C88S , ROGFP2 C151S or ROGFP2 C208S were introduced by PCR with Pfu polymerase using ROGFP2 WT -PFGRX C32S /pET45b or ROGFP2 /pET28a as a template
Moreover, the chemical nature of the C-terminal residue side chain determines ligand contact with RAMP and can influence activity at AMYRs and CTR 18,19