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The eluent was incubated with 150 L 2 M urea, 150 L 1 mM CaCl 2 , and 1 L trypsin (1 g/L) at 37C overnight

After euthanizing the mice, brain tissues were carefully dissected and immersed in a fixative solution for electron microscopy at 4 C for four hours

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Yet they're sedentary where they're constantly moving, they're constantly foraging, they're constantly living in nature instead of going from one box to another box, from our car door home to our office under these blue light exposure

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