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Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32

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May 7, 2020

Caloric restriction has also been shown to alter circulating amino acid profiles and protein turnover dynamics [86, 87], further influencing systemic metabolic regulation under conditions of reduced nutrient inflow