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You can fill the bucket with ICE or freeze 2 two liter soda bottles and drop those in the bucket

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In ajowan ( Trachyspermum ammi L.), a medicinal plant, different levels of gene transformation parameters, including the Agrobacterium optical density (OD), Agrobacterium strain, Agrobacterium killing antibiotic, acetosyringone concentration, and inoculation duration were assessed during the introduction of the BADH gene, and greatest gene transformation efficiency was obtained using the LB4404 strain of Agrobacterium at OD 600 = 0.60.8 160 mg/L timentin 250 mol/L acetosyringone 30 min inoculation duration (Niazian et al., A