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In this study, one group of rats (320 2 g, n = 12) received an injection of vehicle (saline) and after 15 min, an injection of fentanyl

For example, if your protocol calls for 250mcg daily for 30 days, and you reconstituted with 2 mL of bacteriostatic water, each 0.1 mL withdrawal contains a specific amount

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This technique enables not only reduction of matrix effects but also enrichment of studied samples, which is important because of very low concentrations of the pharmaceutical in the environment