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The plasmid was linearized by the restriction enzyme (Thermo Fisher, Waltham, MA, USA) and used as the template to produce mRNA with T7 High Yield RNA Transcription kit (#DD4202, Vazyme)

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doi:10.1016/S1474-4422(18)30403-4 3

Secretins and similar diabetes medications represent the largest increase in drug spending in Canada in 2023.i Diabetes medications are now the top category for drug claims in Canada

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