Aged mice were divided into control and treatment groups, with the latter receiving intraperitoneal injections of FOXO4-DRI at doses of 0.5 mg/mL and 5 mg/kg every other day for 30 days, while controls received PBS
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Approximately 10 mg of tissue from 30 female and male brains were homogenized on ice using a dounce homogenizer in 400 L of 80:20 methanol
Am J Clin Nutr 89:7176 Pignatelli P, Tellan G, Marandola M, Carnevale R, Loffredo L, Schillizzi M, Proietti M, Violi F, Chirletti P, Delogu G (2011) Effect of l-carnitine on oxidative stress and platelet activation after major surgery
Storage between 4 and 18 C is recommended as all recovery rates remained above 76 and 88% after 24 weeks respectively (Osteresch et al