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Using Metformin for weight loss, is not officially approved by health authorities as a weight loss drug, some healthcare providers may recommend the off-label use of Metformin for weight loss purposes

Analysis of brain mitochondria using serial block-face scanning electron microscopy

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In nutrient-rich conditions, mTOR remains active, suppressing autophagy initiation, while under stress conditions such as energy deficiency or DNA damage, the activation of AMP-activated protein kinase (AMPK), p53 or inhibition of pathways like PI3K/Akt and ERK reduces mTOR activity, which then releases the inhibition on autophagy and facilitates its initiation (Yang and Klionsky, 2010)