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Absorbance readings were acquired using a microplate reader (Bio-Rad) at a wavelength of 450 nm

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However, in 2001, a handful of groundbreaking research discoveries confirmed that the Ca 2+ ionophore ionomycin could trigger an apoptosis-like cell death in erythrocytes associated with the appearance of apoptotic signs, including cell shrinkage, membrane vesiculation, or PS exposure [22, 23]

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