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This dose is taken once per week after completing the gradual dose increasing schedule

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Hollowell JG, Staehling NW, Flanders WD, Hannon WH, Gunter EW, Spencer CA, et al

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Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Wash the plate twice before adding the standard and sample