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Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JA
Its encoded protein, circINSIG1-121, recruits the CUL5-ASB6 E3 ligase complex to promote K48-linked ubiquitination of INSIG1a key regulator of cholesterol metabolismat lysine residues 156 and 158, thereby inducing cholesterol synthesis and subsequently facilitating CRC cell proliferation and metastasis (168)
A 2014 publication (McCoy et al., Journal of Pharmacology and Experimental Therapeutics) was retracted due to concerns about data fabrication
In brief, the corresponding sequence (Supplementary Table 2) was cloned and inserted into pFastBacHTA (Supplementary Table 4) and then transformed into DH10Bac (Vazyme, Nanjing, China)-competent Escherichia coli