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The reactions were allowed to incubate in the dark at 37 C for 30 min, during which fluorescence was measured in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) using excitation in the range of 530560 nm and emission detection at 590 nm

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Manufactured under cGMP standards and shipped domestically from Sarasota, FL, this compound is used in in-vitro studies on angiogenesis, epithelial barrier function, and extracellular matrix modulation

Safety Considerations from Published Research Because retatrutide has been investigated in human clinical trials, published literature has described adverse events observed during those studies